Laboratory sterile filtration is essential to reduce potential contamination of cell culture media. Alfa Chemistry provides effective aseptic filtration solutions for laboratories around the world, which will minimize time to market and maximize all development stages.
How Can We Help You？
We provide you with a variety of filters to reduce filtration time, increase throughput, and save filtration costs. Our filter membrane can sterilize liquid reagents and remove particulate contaminants. These products are optimized for biological, pharmaceutical, and sterile filtration requirements, and show superior performance compared to competing filtration devices.
We can help you:
- Maximize product recovery rate
- Reduce filtering time
- Improve throughput
- Reduce the need for multiple filter changes
- Save overall filtration cost
Examples of our strict quality specifications include:
- Gamma radiation sterilization - Products with membranes are pre-sterilized by gamma radiation to eliminate potential cytotoxic residues associated with EtO sterilization.
- Biosafety - The product has passed the U.S. Pharmacopoeia bioreactivity test.
- Pyrogen free -The product uses the Limulus amebocyte lysate test for bacterial endotoxin test to ensure safety.
- Low extractables - The product is optimized to reduce extractables, ensuring that no unwanted materials are introduced into the filtered liquid.
Clarification and Pre-filtration
Filtration is an effective way to reduce particle contamination in fluids. Our filters can be used to clarify high particle load solutions that do not require sterility, such as reagents and buffers. The filter used for clarification or pre-filtration may be a membrane filter with large pore size, or it may be made of a thicker medium with a wider pore size range to capture more particles.
We have a variety of products for clarification and pre-filtration, from small-volume syringe filters to capsule filters that can handle several liters at a time.
Clarification and pre-filtration applications include:
- Fermented broth
- Serum and serum-containing medium
- Compound growth medium
- Industrial and environmental water samples
Removal of Endotoxin
When producing recombinant proteins in E. coli and other Gram-negative bacteria, it is often necessary to remove endotoxins from the final product. A 0.2 μm sterile grade filter is usually used to remove bacteria, but LPS itself is difficult to remove or inactivate because it is very stable to heat and pH. Removal of endotoxin is one of the most difficult downstream processes in protein purification.
We provide effective endotoxin removal solutions. The filter device with a positively charged hydrophilic membrane is ideal for removing endotoxins from the solution due to its highly cross-linked quaternized amine charged surface. This provides very high dynamic capabilities under selected conditions for the removal of endotoxins from process feed streams, buffers and water.
Laboratory Reusable Filter Holder and Membrane
The filter holder and membrane are designed for a variety of applications, including online sterile filtration, stacked sampling, and product sampling. The filter holders selected for sterile filtration usually adopt closed designs, such as syringes or in-line filter holders, which operate most efficiently by using positive pressure to push fluid through the assembled equipment.
Our filter holder can collect the filtrate or easily remove the membrane for further study. The 142 and 293 mm in-line filter holders are especially suitable for small batch tests or the filtration of heavy particulate environmental samples.
Mycoplasma is a very small bacterium characterized by a lack of hard cell walls. Defects in the cell wall cause the organism to have a characteristic elasticity that allows mycoplasma to pass through a 0.2 μm filter. In addition, mycoplasma lacks a cell wall, making it resistant to certain antibiotics, which interfere with the synthesis of bacterial cell walls.
Using our 0.1 μm membrane is your best guarantee that mycoplasma will not be introduced into your culture through serum or serum-containing media. This is an inherently hydrophilic PES membrane that reduces mycoplasma. We incorporate this membrane into many laboratory filtration products that are very suitable for tissue and culture applications.
Sterile Membrane Filtration
Filtration through 0.2 µm pore size is considered necessary to produce sterile filtrate. Typical fluids may include media with or without serum, buffers, biological or protein samples, reagents, or other fluids that require no particle or microbial contamination.
Although sterile filtration is accepted as a 0.2 µm pore size, our comprehensive sterile filter product line includes larger pore sizes, which can be used for samples that would be harmed if contaminated by microorganisms during the use of non-sterile device.
Laboratory vacuum filtration is a simple and convenient method of filtering fluids, which can be produced by pumps, internal vacuum lines, or tap suction systems. Vacuum filter devices, also called bottle-top vacuum filters, are commonly used for sterile media and buffer preparation. We provide a complete set of vacuum filtration devices and bottle-less top filters.
Our sterile vacuum filtration device meets the following important characteristics:
- It fully uses the filtration potential to fill multiple media bottles
- It Improves safety and ergonomics by eliminating repeated lifting and dumping
- By sucking the fluid directly from the mixing bottle into the filter device, the risk of contamination, spillage, and time loss is eliminated.