Lentiviral vectors are one of the most commonly used viral vectors for therapeutic purposes because they have specific functional properties, such as the ability to infect non-dividing cells and packaging capabilities. RNA-based vectors are designed to safely and effectively deliver genes to mammalian cells. Several lentiviral vector-based therapies are now being developed and tested in humans. It is difficult to develop a dependable method for producing lentiviral vector-based therapeutics. Due to their low stability, purification of these 80-120 nm envelope vectors is problematic.
There are a variety of approaches to creating lentiviral vectors, but most of them follow the same fundamental steps with minor variations as needed. Alfa Chemistry has a skilled technical team available at all times to assist you in finding the optimum solution based on data and testing.
Upstream
Adherent cell seeding: A closed disposable device for amplifying shear-sensitive adherent mammalian cells under regulated conditions is the Multiplate Bioreactor.
Adherent cell culture: We provide cell density bioreactors with integrated disposable technology to simplify processes involving adherent cells while also ensuring process safety.
Suspension cell culture: XRS bioreactor system is a disposable bioreactor system with unique agitation and control characteristics, specially designed for the suspension culture of mammalian cells under controlled conditions.
Clarification: This step is ideal for our filter membrane, which comes in a variety of sizes and qualities. For assistance in determining the optimum filtering technology for your process, contact us.
Downstream
Purification (Chromatography): Expandable membrane chromatography capsules are a flexible option that lowers the consumption of buffers and shortens processing time as compared to typically packed bed resins.
Ultrafiltration/Diafiltration (UF/DF): Disposable tangential flow filtration modules using polyethersulfone (PES) membranes can be used in disposable TFF settings as ready-to-use gamma irradiation cassette modules.
Bulk Drug Substance Filtration: Membranes such as EKV, EX ECV, and EX EDF are suited for this stage and come in a variety of sizes and characteristics. For assistance in determining the optimum filtering technology for your process, contact us.