Researchers are under pressure to reduce the cost of drug development. The key to improving R&D efficiency is to quickly generate clonal cell lines that can provide high concentrations of high-quality recombinant protein, and to screen out failed candidate clones as soon as possible.
Alfa Chemistry provides laboratory solutions to support researchers throughout the cell line development and clone selection process. High-performance membranes and innovative centrifugal devices solve the challenges of DNA/RNA and protein sample preparation. The high-tech filtration system is designed to maintain sterility while simplifying the harvest, clarification, and purification of cell culture products for analysis.
Cell Line Development
The development of high-yield and stable cell lines is the basis for the production of biotherapeutics, such as monoclonal antibodies, bispecific monoclonal antibodies, fusion proteins, and vaccines. Alfa Chemistry provides solutions to support researchers throughout the cell line development process as a global leader in high-tech filtration and purification systems. High-performance proprietary membranes solve the challenges of complex next-generation biologics while supporting expansion into commercial manufacturing processes. Innovative and time-saving products for filtration, separation, and sample preparation help simplify workflow and ensure sterility from gene editing, cell culture to omics analysis.
Gene Editing
The multi-step gene editing workflow is resource-intensive and involves gene selection, PCR amplification, DNA extraction, etc. Always be vigilant to avoid contamination and downstream complications. Alfa Chemistry centrifugal ultrafiltration unit simplifies many common nucleic acid preparation procedures.
Restriction digestion of plasmid DNA and DNA amplification products can be carried out in a centrifuge. The connection of DNA fragments to the vector can also be done directly in the ultrafiltration device so that the product is ready to be transformed into competent cells.
Nucleic acid-binding (NAB) products provide flexibility, reduce the risk of cross-contamination, and smooth sample handling. NAB products are available in the form of a single-tube centrifuge device and a high-throughput 96-well filter plate, providing researchers with the flexibility to purify plasmid DNA, genomic DNA, or total RNA in a single device.
Strain Structure
Effective strain construction by transforming bacterial cells or transfecting yeast or mammalian cells requires effective recovery, concentration, and delivery of target nucleic acid fragments. The preparation of a small volume medium for transformation or transfection requires specific strain constructs with amino acid or antibiotic changes. When dealing with small volumes of media that cannot be autoclaved, a sterile syringe filter can help you maintain sterility.
Our laboratory's 0.2 µm sterile filters and capsules ensure that liquid media are effectively filtered to eliminate the risk of contamination. When it is necessary to further concentrate constructs before introducing cells, our extensive low molecular weight cut-off (3 to 1000 kDa) ultracentrifugation devices and plates simplify sample handling and preparation.
Cell Culture
Aseptic technique and sterility in cell culture are essential to avoid contamination and downstream complications. In addition to ensuring sterile or aseptic culture conditions, the culture medium and nutrient feed also needs to be filtered and sterilized. It is also necessary to avoid cross-contamination when handling cell lines.
Alfa Chemistry's low protein binding 0.2 µm filter media provides fast and effective filtration to maintain sterility in cell culture applications. The proprietary double-layer membrane can sterile filter nutrient-rich media and supplements while ensuring that valuable nutrients are not lost. Our 0.1 µm membrane is an inherently hydrophilic polyethersulfone (PES) membrane that traps mycoplasma and provides the best protection against mycoplasma contamination in serum or serum-containing media for cell culture.
Clone Screening
Maintaining sterility and clonality is important for the purity and homogeneity of the target protein. It is essential to screen a large number of clones as soon as possible to select valuable clones for further analysis. In addition to sterile filtration systems that ensure sterility of the culture medium and environment, Alfa Chemistry also provides centrifugal devices and filter plates to simplify protein sample preparation, gDNA, and mRNA purification.
The filter plate is designed to reduce the risk of cross-contamination and to create a smooth liquid flow for sample processing and high-throughput screening. The high-sensitivity protein binding membrane designed for protein detection and analysis applications has low background noise to ensure the clarity and sensitivity of the results.
Sample Preparation for Omics Analysis
Simplifying these processes through efficient sample preparation and purification of biomolecules for analysis can save a lot of time and resources.
- We provide filtration devices with membranes that can effectively remove endotoxins.
- Our high-performance depth filter media can effectively remove cells and clarify cell lysates, and has a low hold-up, thereby improving product recovery.
- The simple, easy-to-use, innovative nucleic acid binding centrifugation device uses double-layer glass fiber membranes to provide high yield and flexibility. It can purify genomic DNA and mRNA in a single device to support multiple downstream applications.
- We provide highly sensitive protein binding membranes designed for protein detection and analysis using Western blotting and other techniques.