The emergence of next-generation sequencing technology has made it possible to collect unprecedented amounts of sequencing data in a short period. This has greatly promoted our understanding of the genome and gene structure of many species, and has also allowed us to obtain a wealth of information about genetic diseases, cancers that cause somatic mutations, and misexpressed candidate genes caused by epigenetic changes.
Alfa Chemistry aims to plan your success. We provide you with DNA and RNA purification solutions to remove particles quickly and effectively. More of the workflow involved in the research is performed in a high-throughput manner through the use of porous DNA binding filter plates, which allow more genomic DNA samples to be obtained in parallel.
Total RNA Purification
Transcription of genes into RNA is an important step in the synthesis of functional gene products. Alfa Chemistry provides a 96-well long tip filter plate for nucleic acid binding containing silica-based quartz glass fiber media, which can effectively bind DNA and RNA, while providing smooth flow and rapid sample processing. In addition, our new long tip plate minimizes the formation of hanging drops, thereby reducing the possibility of cross-contamination.
Genomic DNA Purification
The emergence of next-generation sequencing technology has made it possible to collect unprecedented amounts of sequencing data in a short period of time. More of the workflow involved in this study was performed in a high-throughput manner through the use of porous DNA binding filter plates, which allow more genomic DNA samples to be obtained in parallel.
We provide 96-well long tip filter plates containing silica-based quartz glass fiber media, providing researchers with the flexibility to purify plasmid DNA from bacteria and genomic DNA or total RNA from cell culture samples - one plate is available For multiple applications.
Additional purification methods can include a chromatographic step. We can provide a new chromatographic membrane that can provide higher dynamic binding capacity. Our chromatographic membranes help to remove host cell proteins and are dedicated to purifying genomic DNA. They are easy to use and disposable, creating the best sterile environment for purification using ion-exchange chromatography.
DNA / RNA Plasmid Purification
Plasmid DNA plays a key role in molecular biology research, but obtaining it from prokaryotic cultures can be tricky. It needs to be separated from the genomic DNA in a complex lysis and purification process.
The products we provide allow plasmid DNA to be obtained from prokaryotic cultures. The amount of plasmid DNA retained is higher than that in prepackaged samples, and the cost is lower. Using our 96-well filter plate for DNA purification for lysate removal, the lysate sample preparation procedure can effectively remove unwanted cell debris from the sample.
Northern and Southern Blotting
Northern and Southern blotting are standard molecular biology techniques for RNA and DNA identification and quantification, respectively. The analysis method is based on the immobilization of nucleic acid. Alfa Chemistry provides transfer membranes with high sensitivity, low background, and batch-to-batch consistency for all radioactive and non-radioactive detection methods.
For Northern and Southern blotting solutions, please click here.