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Northern and Southern Blotting

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Casein Blocking Buffer 10x for Northern and Southern Blotting

Cat.No   ACMA00032644
This product is a casein-based blocking agent, usually used to reduce non-specific binding of detection reagents to Northern and Southern Blot membranes.
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Casein Blocking Buffer for Northern and Southern Blotting

Cat.No   ACMA00032645
This product is commonly used in blocking buffers for Western, Southern and Northern Blot procedures. The blocking agent is used to reduce the non-specific binding of the detection reagent to the membrane.
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Denaturation Solution for Northern and Southern Blotting

Cat.No   ACMA00032646
This product is a strong base that can denature double-stranded DNA so that it can be efficiently transferred to a nylon membrane and then hybridized with the labeled probe.
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Denhardt's Solution for Northern and Southern Blotting

Cat.No   ACMA00032647
This product is a blocking agent, usually used to reduce non-specific binding of detection reagents to Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration and used in a variety of pre-hybridization and hybridization buffers.
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Gelatin Blocking Buffer for Western Blotting

Cat.No   ACMA00032648
This product is the standard reagent in Western blotting procedures. When nylon or nitrocellulose is used, it is used to block non-specific binding on the membrane.
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Hybridization Buffer for Northern and Southern Blotting

Cat.No   ACMA00032649
This is a ready-to-use hybridization buffer for Northern and Southern blot protocols. The use of this product can decrease hybridization time to as little as two hours.
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Hybridization Solution for Northern and Southern Blotting

Cat.No   ACMA00032650
This product is a 2x ready-to-use hybridization buffer, suitable for Northern and Southern blotting protocols.
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Neutralizing Buffer for Northern and Southern Blotting

Cat.No   ACMA00032651
The neutralization buffer of this product used in the Southern procedure will neutralize the pH of the gel after depurination and denaturation.
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Prehybridization Solution for Northern and Southern Blotting

Cat.No   ACMA00032652
This product is a 2x ready-to-use buffer, suitable for Northern and Southern blotting protocols.
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SSC Buffer 20× Concentrate for Northern and Southern Blotting

Cat.No   ACMA00032653
This product is the standard reagent in Southern and Northern hybridization procedures. It is used to control the stringency of the washing buffer in the washing step after hybridization.
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SSC Buffer 20x for Northern and Southern Blotting

Cat.No   ACMA00032654
This product is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used directly or diluted to make an SSC working solution with a concentration as low as 0.5 times.
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SSC Buffer for Northern and Southern Blotting

Cat.No   ACMA00032655
This product is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be re-dissolved in molecular biology grade water to the specified final volume (20 times the final solution) before use.
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SSPE Buffer 20× Concentrate for Northern and Southern Blotting

Cat.No   ACMA00032656
This product is the standard reagent in Southern and Northern hybridization procedures. This liquid can be used directly or diluted as needed.
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Tris Acetate-EDTA Buffer Concentrate for Northern and Southern Blotting

Cat.No   ACMA00032657
This product is often used for nucleic acid electrophoresis. The buffering capacity of this solution is lower than that of TBE buffer, but double-stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted to 1x with molecular biology grade water before use.
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Tris-Borate-EDTA Buffer solution for Northern and Southern Blotting

Cat.No   ACMA00032658
is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
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Tris-Borate-EDTA Buffer Substancefor Northern and Southern blotting

Cat.No   ACMA00032659
It is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble and protected from degradation. This powder should be reconstituted in molecular biology grade water before use.

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