Cat.No ACMA00032634
- Description
- Application
- Features And Benefits
This product provides a simple and convenient method to isolate pure genomic DNA from fresh or old (more than 24 hours) whole blood. No need for expensive resins, alcohol precipitation, and dangerous organic compounds such as phenol and chloroform.
Restriction enzyme digestion; polymerase chain reaction; southern blotting; sequencing reaction; cloning
1. Starting material: up to 200 μl of fresh or stale blood
2. Expected yield: up to 10 μg
3. Elution volume: 400 μl
4. Time required: <40 minutes
5. Compatible with a variety of anticoagulants, including EDTA, heparin and sodium citrate
| Pack Size | 1 KIT |
|---|---|
| Preparation Note | The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length. |
| Usage | sufficient for 350 purifications |