Cat.No ACMA00032641
- Description
- Application
- Features And Benefits
This product provides a simple and convenient method to isolate pure high molecular weight DNA from various mammalian sources. These kits use silica gel membranes, which are specifically selected for genomic DNA purification, and use a convenient spin column format. There is no need for expensive resins, alcohol precipitation, and dangerous organic compounds such as phenol and chloroform. "
Extract DNA from fin samples; extract BSTUI restriction endonuclease from DNA; separate DNA from blood samples extracted from caudal fin; extract DNA from tissues and hair follicles
1. Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg tissue
2. Elution volume: 200-400 μl
3. Time required: 20 minutes after lysis
| Synonyms | Mammalian Genomic DNA Miniprep |
|---|---|
| Pack Size | 1 KIT |
| Preparation Note | The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length. |
| Usage | sufficient for 70 purifications |