Cat.No ACMA00032661
- Description
- Application
- Features And Benefits
This product provides a simple and convenient method to isolate total RNA from mammalian cells and tissues. The kit combines the advantages of a silica-based system and a microcentrifugation format, eliminating the need for cesium chloride gradients, alcohol precipitation, and harmful organic compounds such as phenol and chloroform.
The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications.
1. Up to 107 cells or 40 mg of tissue can be purified for total RNA per preparation; up to 150 μg of pure concentrated total RNA can be produced per preparation
2. Simple and efficient-12 to 18 preparations in 30 minutes, faster than the gravity flow anion exchange method
| Synonyms | Mammalian RNA Kit |
|---|---|
| Component | Mammalian Total RNA Purification Kit combines silica-membrane technology with a convenient spin column format for a rapid bind, wash, and elute method to prepare high quality total RNA. |
| NACRES | NA.52 |
| Pack Size | 1 KIT |
| Preparation Note | Samples are lysed and homogenized in guanidine thiocyanate and 2-mercaptoethanol to release RNA and inactivate RNases. Lysates are spun through a filtration column to remove cellular debris and shear DNA. The filtrate is then applied to a high capacity silica column to bind total RNA, followed by washing and elution. Up to 150 μg of total RNA can be recovered per prep in 100 μl of water. The purified RNA is ready for Northern blots, RT-PCR and other common applications. |
| Usage | sufficient for 70 purifications |