- Description
- Application
Hydrophobic interaction chromatography (HIC) separates and purifies biomolecules based on differences in their hydrophobicity. The phenyl membrane adsorber follows the same rules known from the conventional hydrophobic interaction chromatography. Due to the large pore size, membrane adsorbers show excellent flow properties. There is almost no diffusion limitation of mass transport compared with conventional bead chromatography. Buffers with high concentrations of salt promote the adsorption of proteins on the hydrophobic membrane matrix. Proteins are eluted by decreasing the salt concentration in the elution buffer.
This product can be used to remove: aggregates, host cell proteins, viruses, endotoxins; lipids, dyes, detergents and defoamers; leaching chromatography ligands
| Density | Ligand 3 µeq/cm2Membrane |
|---|---|
| Area | 0.7 cm2Membrane |
| Bed Volume | 19 µL/well |
| Binding Capacity | Typical Dynamic Binding Capacity (10% per unit) 0.28 mg/well |
| Height | Bed 0.8 mm |
| Material | Housing Polypropylene (PP) Material 8-strip Polypropylene (PP) Holding Frame Polystyrene Membrane Stabilised reinforced cellulose Construction Polyester |
| Pack Size | 10 |
| pH | Stability 2 - 14 (short term) |
| Pore Dimensions | 3 - 5 µm |
| Pressure Range | Max 35 kPa |
| Reference Protein | Gamma globulin from bovine blood |
| Type | (Membrane) Hydrophobic interaction chromatography membrane (HIC) |